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Jn-Mini Low Temperature Ultra High Pressure Continuous Flow Cell Disrupter

  • Price:

    Negotiable

  • minimum:

  • Total supply:

  • Delivery term:

    The date of payment from buyers deliver within days

  • seat:

    Guangdong

  • Validity to:

    Long-term effective

  • Last update:

    2017-11-01 01:23

  • Browse the number:

    96

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Company Profile

Guangzhou Juneng Nano&Bio Technology Co., Ltd.

By certification [File Integrity]

Contact：Miss Su Ning(Mr.)  

Email：

Telephone：

Area：Guangdong

Address：Guangdong

Website： http://jnbio-homogenizer.liyangyacht.com/

Product Details

Part 1 Key technical parameters and Specifications
 
Pressure 0-207MPa (2070bar)
Flow rate under max. pressure 1.2L/hr (20ml/min)
Min.sample treatment capacity 3ml
Power supply 220V, main unit 0.75kW + cooling 0.45kW
Whole preparation and dispersion process completed in a water bath Using ice-water mixture
Whole preparation and dispersion process completed in a 4-6ºC water bath Low-temperature circulating water bath to be purchased separately
Item Qty. Power/ voltage (kW/V) External dimensions (mm)
Length (L) Width (W) Height (H)
Main unit 1 0.75/220 630 450 355
Cooling system 1 0.45/220 603 405 409

Part 2 Purposes
Suitable for use by colleges, universities, research institutes, high-tech and biomedicine companies, biological product manufacturers, etc.
  1. Cell disruption of bacteria (e.g., Escherichia coli), fungi (e.g., yeast/Ganoderma lucidum spores), and alga (e.g., blue/green alga);
  2. Cell disruption of animals and plants (e.g., nematodes);
  3. Cell disruption of actinomycetes, thermophiles, and mycobacteria tuberculosis;
  4. Enzyme preparation and separation, and preparation of recombinant polypeptides;
  5. Crystal engineering, and protein crystals;
  6. Nanometer emulsification and homogenization of emulsions, liposomes, etc.; micro-emulsions, and nano-suspensions;
  7. Disruption and extraction of Chinese medicinal herbs;
  8. Ultra-high pressure induced mutation breeding;
  9. Grinding and dispersion of nanometer materials, paints, inks and dyes, including graphemes, carbon nano-tubes, carbon nano-powders and nano high-molecular materials
Part 3  Principle

Low-temperature ultra-high pressure continuous flow cell disrupters use ultra-high pressures to release the sample instantly via a slit, and disrupt cells under the action of the shearing, cavitation and collision effects, so that materials are homogenized, dispersed, emulsified and micronized. The whole process is performed in a 4-6ºC low-temperature circulating water bath, preserving original activity and performance.


Fig.1 The principle diagram of the disruption Fig.2 Main unit immersed in a circulating water bath 

 

Part 4  Features
  1. Exclusive cell disrupters in the world in which the whole process of sample feeding, disruption and sample release is 4-6ºC low-temperature circulating water bath, preserving original activity and performance (optional circulating cooler)
  2. Suitable for research and continuous production
  3. World-class pressure rating of 207MPa (2070bar), largely meeting user requirements
  4. Min. sample treatment capacity: JN-Mini (3ml), JN-02C (5ml)
  5. Diamond disruption valve with wear resistance tens of or even nearly 100 times of porcelain or tungsten carbide
  6. Hydraulic main power, capable of free shutdown, starting and pause under high pressures while maintaining pressure
  7. Capable of no-load operation, being vented by just pressing with a stainless steel needle gently after charge without changing pressure (patented technique)
  8. Food- and pharmaceutical-grade advanced pollution-free materials
  9. In-situ cleaning and disinfection
  10. High-strength full-stainless steel ultra-high pressure pump
  11. Physical disruption, eliminating chemical residue
  12. All containers and pipes through which the sample is disrupted made of 316L stainless steel with excellent resistance to pollution and corrosion
  13. Excellent safety, with the hydraulic disruption valve serving as a sensitive safety valve
  14. Easy pressure regulation without handling the spring hard
  15. Metal sealing, without any rubber seal
  16. Independent intellectual property rights and core techniques, with ease of maintenance by widespread engineers
 
Part 5  Selected application samples
 
Protein gel electrophoretogram of cell disruption of Escherichia coli (Fig. 3)
Disruption rate of yeasts and Escherichia coli up to 95%-99% (Fig.4 , 5)

 

 

Fig.4 Yeast cells before disruption Fig.5  Yeast cells after disruption